Search results for "genetic manipulation"
showing 3 items of 3 documents
Technologies for cultivation and exploitation of microalgae in industrial applications
2020
This thesis work is focused on technologies for the development of the microalgal industry. During this 3-years PhD work several themes were addressed. Section 1 consists in a literature review concerning the general features of microalgae, their composition and a description of the photosynthetic process. Furthermore, themes such as biosynthetic pathways, growth kinetics, microalgae cultivation and their applications are reviewed. Section 2 focuses on the genetic manipulation of microalgae aimed at producing a vitamin B12-enriched food supplement. In this case, the human intrinsic factor gene, was inserted in the genome of the microalgae Chlamydomonas reinardhtii in order to integrate new …
Genetic manipulation of spiramycin-producing strain Streptomyces ambofaciens ATCC 23877 by φC31 Att/Int system-based vectors results in a significant…
The genus Streptomyces produces about two-thirds of naturally occurring antibiotics and many other biologically active secondary metabolites. The genetic manipulation of Streptomycetes is often labor and time intensive due to their large genome and complex development. Much progress has been made to develop gene transfer methods useful to construct antibiotic-producing strains with improved properties. The φC31 Att/Int system is an integration system that has been widely used to produce stable recombinants in Actinomycetes and its integration site is an attB site located in a pirin-like gene (pirA) of the bacterial chromosome. In this study we demonstrate that the integration of φC31 DNA in…
Jürgen Habermas and the risk of liberal eugenics for the ethical self-understanding of species
2016
El artículo analiza el problema de la naturaleza humana que presenta Jürgen Habermas en su obra El futuro de la naturaleza humana, ¿Hacia una eugenesia liberal? Los vertiginosos avances biotecnológicos reclaman una rigurosa reflexión bioética que el filósofo alemán afronta superponiendo a su ética del discurso una ética de la especie. Habermas esboza un modelo de naturaleza humana construido sobre preceptos como la dignidad humana y de la vida humana, la simetría de las relaciones intersubjetivas y, a resultas de estas, la autocomprensión ética de la especie. Respecto a la eugenesia liberal, el autor se posiciona criticando la eugenesia positiva y amparando la negativa, para lo que basa su …